RESUMEN
Syntaxin-1A (stx1a) repression causes a neurodevelopmental disorder phenotype, low latent inhibition (LI) behavior, by disrupting 5-hydroxytryptaminergic (5-HTergic) systems. Herein, we discovered that lysine acetyltransferase (KAT) 3B increases stx1a neuronal transcription and TTK21, a KAT3 activator, induces stx1a transcription and 5-HT release in vitro. Furthermore, glucose-derived CSP-TTK21 could restore decreased stx1a expression, 5-HTergic systems in the brain, and low LI in stx1a (+/-) mice by crossing the blood-brain barrier, whereas the KAT3 inhibitor suppresses stx1a expression, 5-HTergic systems, and LI behaviors in wild-type mice. Finally, in wild-type and stx1a (-/-) mice treated with IKK inhibitors and CSP-TTK21, respectively, we show that KAT3 activator-induced LI improvement is a direct consequence of KAT3B-stx1a pathway, not a side effect. In conclusion, KAT3B can positively regulate stx1a transcription in neurons, and increasing neuronal stx1a expression and 5-HTergic systems by a KAT3 activator consequently improves the low LI behavior in the stx1a ablation mouse model.
Asunto(s)
Proteína p300 Asociada a E1A , Sintaxina 1 , Animales , Ratones , Modelos Animales de Enfermedad , Histona Acetiltransferasas/metabolismo , Histona Acetiltransferasas/genética , Ratones Endogámicos C57BL , Ratones Noqueados , Neuronas/metabolismo , Fenotipo , Serotonina/metabolismo , Sintaxina 1/metabolismo , Sintaxina 1/genética , Lisina Acetiltransferasas/metabolismo , Proteína p300 Asociada a E1A/metabolismoRESUMEN
The indiscriminate use of pesticides leads to irreparable damage to the ecosystem, which motivates for sustainable alternatives like pheromone-assisted pest management. The tomato pinworm Tuta absoluta is a major threat to tomato cultivation. Moreover, its green management technology uses a pheromone trap that has a short field life. To overcome this problem, a pheromone composite with graphene oxide (GO) and amine-modified graphene oxide (AGO) that can extend the diffusion path has been developed. The composite stimulates an effective electrophysiological response in the antenna, which results in trapping of a significantly higher number of insects as compared to the commercial septa, thus qualifying it for field evaluation. Compared to AGO, the GO composite has pheromones assembled into a multilayer, which increases the pheromone diffusion path. This in turn resulted in the extension of the pheromone life that proportionally increased the pest trapped. This technique will be beneficial to farmers as they have longer field efficacy to keep the pest damage low in an environmentally friendly manner.
Asunto(s)
Acetatos/farmacología , Preparaciones de Acción Retardada/química , Alcoholes Grasos/farmacología , Grafito/química , Control de Insectos/métodos , Nanocompuestos/química , Feromonas/farmacología , Animales , Masculino , Mariposas Nocturnas/efectos de los fármacosRESUMEN
A room-temperature sol-gel-based process was used to produce by direct synthesis talc-like organosilicates having hexadecyl or aminopropyl groups pending in the interlayer space. Thermal analyses, Fourier transform infrared and 13C/29Si solid-state nuclear magnetic resonance spectroscopies confirmed the presence of organic moieties bonded to the inorganic network. Exfoliation of these organoclays in polar solvents such as water for the positively charged magnesium phyllo(aminopropyl)silicate, and in low polar solvents such as toluene and chloroform for hydrophobic magnesium phyllo(hexadecyl)silicate, was investigated by TEM. The ability of these layered magnesium organosilicates to exfoliate in appropriate solvents was exploited for the preparation of transparent self-supporting films and ordered macroporous networks using by latex colloidal crystal templates.
